Reverse Phase Hplc Method for Determination of Nebivolol in Pharmaceutical Preparations

A simple and reliable high-performance liquid chromatography (HPLC) method was developed and validated for determination of nebivolol in pharmaceutical preparations. The method was developed on Ace C18 column using a mobile phase of methanol-water (70:30, v/v). The eluent was monitored by UV detection at 282 nm. The analysis was performed in less than 6 min with a flow rate of 1.0 mL min. Calibration curve was linear over the concentration range of 0.25-8.0 μg mL. Intraand inter-day precision values for nebivolol were less than 4.82, and accuracy (relative error) was better than 3.00%. The mean recovery of nebivolol was 99.8% for pharmaceutical preparations. The limits of detection (LOD) and quantification (LOQ) were 0.10 and 0.25 μg mL, respectively. Also, the developed and validated HPLC method was successfully applied for the quality control of commercial nebivolol dosage forms to quantify the drug and to check the formulation content uniformity.